Increasingly nanoscale particles, pores and surfaces, are being tailor-made to provide high performance separation and detection. New superficially porous (core shell) silica particles for liquid chromatography are a good example in separation science, where nanoscale control over shell thickness enhances column efficiency. Nanoparticle incorporation in the separation and/or sensing roles, can also be an attractive means of enhancing selectivity and detectability. In this work, sensitive and selective detection of disease indicating biomarkers is demonstrated using capillary electrophoresis with embedded gold and silica nanoparticles and amperometric detection at a boron doped diamond (BDD) electrode. The BDD electrode has emerged to play an important role in improving sensitivity in electrochemical detection. Luong et al.1 reviewed the application of the BDD electrode for sensitive detection in bioanalysis. Chen et al.2 reviewed the application of the BDD electrode for electrochemical detection in capillary electrophoresis.By coating the fused silica capillary with gold nanoparticles (AuNPs) embedded in a cationic polymer poly (diallyldimethylammonium) chloride (PDDA), efficient separation and sensitive detection was achieved for analysis of 3-indoxyl sulphate (IXS), vanillylmandelic acid (VMA), homovanillic acid (HVA) and tryptophan (TRP) in urine3. The application of this separation and sensing approach is further applied in the field of bacterial cell-to-cell communication in the analysis of cell signalling molecules in the Pseudomonas Quinolone Signal (PQS) quorum sensing system4. P. aeruginosa is best known as an antibiotic resistant human pathogen associated with hospital-acquired infections and is the primary cause of morbidity and mortality in cystic fibrosis sufferers.
References:
1. J.H.T. Luong, K.B. Male, J.D. Glennon, Analyst, 2009, 134, 1965.
2. G. Chen, Talanta, 2007, 74, 326.
3. L. Zhou, J.D. Glennon, J.H.T. Luong, Anal Chem, 2010, 82, 6895.
4. L. Zhou, J.D. Glennon, F.J. Reen, F. O’Gara, C.M. McSweeney, G.P. McGlacken, Chem Comm, 2011, 47, 10347.
Presenting author:
Jeremy D. Glennon
Irish Separation Science Cluster (ISSC), Department of Chemistry and the Analytical &Biological Chemistry Research Facility (ABCRF), University College Cork, Western Road, Cork, Ireland
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Authors:
Jeremy D. Glennon - Irish Separation Science Cluster (ISSC), Department of Chemistry and the Analytical &Biological Chemistry Research Facility (ABCRF), University College Cork, Western Road, Cork, Ireland
Alyah Buzid - Irish Separation Science Cluster (ISSC), Department of Chemistry and the Analytical &Biological Chemistry Research Facility (ABCRF), University College Cork, Western Road, Cork, Ireland
Phyllis E. Hayes - Irish Separation Science Cluster (ISSC), Department of Chemistry and the Analytical &Biological Chemistry Research Facility (ABCRF), University College Cork, Western Road, Cork, Ireland
Gerard P. McGlacken - Department of Chemistry and the Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland
John H.T. Luong - Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, Canada H4P2R2
